• Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms 

      Lindivat, Mathilde; Larsen, Aud; Hess-Erga, Ole-Kristian; Bratbak, Gunnar; Hoell, Ingunn (Peer reviewed; Journal article, 2020)
      In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active ...
    • Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment 

      Lindivat, Mathilde; Bratbak, Gunnar; Larsen, Aud; Hess-Erga, Ole-Kristian; Hoell, Ingunn Alne (Peer reviewed; Journal article, 2021)
      Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT–SYBR ...
    • Patterns in Microbial Assemblages Exported From the Meltwater of Arctic and Sub-Arctic Glaciers 

      Kohler, Tyler J.; Vinšová, Petra; Falteisek, Lukáš; Žárský, Jakub D.; Yde, Jacob C.; Hatton, Jade E.; Hawkings, Jon R.; Lamarche-Gagnon, Guillaume; Hood, Eran; Cameron, Karen; Stibal, Marek (Peer reviewed; Journal article, 2020)
      Meltwater streams connect the glacial cryosphere with downstream ecosystems. Dissolved and particulate matter exported from glacial ecosystems originates from contrasting supraglacial and subglacial environments, and ...
    • Spatial Variability of Antarctic Surface Snow Bacterial Communities 

      Malard, Lucie A.; Sabacka, Marie; Magiopoulos, Iordanis; Mowlem, Matt C.; Hodson, Andrew J.; Tranter, Martyn; Siegert, Martin J.; Pearce, David A. (Journal article; Peer reviewed, 2019)
      It was once a long-held view that the Antarctic was a pristine environment with low biomass, low biodiversity and low rates of microbial activity. However, as the intensity of scientific investigation has increased, so ...