Browsing HVL Open by Author "Bratbak, Gunnar"
Now showing items 1-2 of 2
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Bioorthogonal Non-canonical Amino Acid Tagging Combined With Flow Cytometry for Determination of Activity in Aquatic Microorganisms
Lindivat, Mathilde; Larsen, Aud; Hess-Erga, Ole-Kristian; Bratbak, Gunnar; Hoell, Ingunn (Peer reviewed; Journal article, 2020)In this study, we have combined bioorthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM) analysis, and we demonstrate the applicability of the method for marine prokaryotes. Enumeration of active ... -
Flow Cytometric Analysis of Bacterial Protein Synthesis: Monitoring Vitality After Water Treatment
Lindivat, Mathilde; Bratbak, Gunnar; Larsen, Aud; Hess-Erga, Ole-Kristian; Hoell, Ingunn Alne (Peer reviewed; Journal article, 2021)Bacterial vitality after water disinfection treatment was investigated using bio-orthogonal non-canonical amino acid tagging (BONCAT) and flow cytometry (FCM). Protein synthesis activity and DNA integrity (BONCAT–SYBR ...